Hyperspectral imaging (HSI) can make the invisible visible. With the special method SERDS (shifted excitation Raman difference spectroscopy), for example, non-destructive real-time characterization of biological tissue can be carried out. Raman signals provide precise information that is not only molecule-specific, but also unique to the type of sample under investigation. For biological materials such as proteins, carbohydrates, lipids, nucleic acids and deoxyribonucleic acids (DNA), the molecular structures are similar but different in detail. Due to their biological function in the body, these functional substances reveal different disease patterns, tissue types and pathogens. Raman spectra can be used, for example, to clearly detect the presence of cholesterol, cancer cells or biomarkers and make them locally visible.